Skip to main content
. 2020 Oct 9;20(6):171. doi: 10.3892/etm.2020.9301

Figure 2.

Figure 2

HIPK2 knockdown suppresses IL-6 and TNF-α production in macrophages. (A) Knockdown efficacy of si-HIPK2 and controls at the mRNA level in LPS-stimulated PMs at the indicated time points (n=3). (B) Western blot analysis of HIPK2 following siRNA interference, and levels of p-p65 and p65 in PMs (three independent repeated experiments were performed). ELISA analysis of (C) IL-6 and (D) TNF-α in the supernatant following HIPK2 knockdown in LPS-stimulated PMs (n=4). *P<0.05 vs. control; #P<0.05 vs. corresponding si-controls in the same group. HIPK2, homeodomain-interacting protein kinase 2; si, small interfering RNA; PMs, peritoneal macrophages; p-, phosphorylated; LPS, lipopolysaccharides.