Figure 7.

Knockdown of KRT80 expression decreases the expression levels of cell cycle-related proteins. SW480 cells were transfected with siRNA-KRT80 or siRNA-NC for 48 h. (A) Western blotting was used to analyze the expression levels of KRT80, PPP1CA, p21 and p27. (B) Semi-quantification of the western blotting data in part A. β-actin was used as the internal loading control for the western blotting analysis. Data are presented as the mean ± SD from 3 independent experimental repeats. ^*P<0.05, ^**P<0.01 vs. siRNA-NC group. KRT80, keratin 80; PPP1CA, protein phosphatase 1 catalytic subunit α; p21, cyclin-dependent kinase inhibitor 1A; p27, cyclin-dependent kinase inhibitor 1B; siRNA, small interfering RNA; NC, negative control.