Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 21;15:61. doi: 10.1186/s13024-020-00411-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — hiBFCNs retain aging-associated features. a Conversion efficiency for the indicated human fibroblast samples analyzed at 14 dpi. hiBFCNs were derived from young (2–3 years) and old (47–79 years) samples and co-cultured with mouse astrocytes (mean ± SEM; n = 3717 GFP⁺ cells for Young samples; n = 10,323 GFP⁺ cells for NL samples; and n = 8752 GFP⁺ cells for AD samples). b Quantification of primary neurite numbers per neuron for the indicated samples at 51 dpi (mean ± SEM; n = 361 cells for Young and n = 1052 for Old samples; *p = 0.0175). c Primary neurite numbers per neuron for the indicated samples at 51 dpi (mean ± SEM; n = 875 for NL and n = 538 for AD samples; ns, not significant). d Representative confocal images for marker expression in the indicated samples co-cultured with astrocytes at 51 dpi. The profiles of DAPI⁺ nuclei are outlined. Scale bar, 10 μm. e Quantifications of marker expression for the indicated young or old samples. Each dot represents a single cell (mean ± SEM; γH2AX: n = 426 for Young, n = 1263 for Old, ****p < 0.0001; HP1γ: n = 139 for Young, n = 421 for Old, ****p < 0.0001; H3K9me3: n = 146 for Young, n = 243 for Old; *p = 0.0446). f Quantifications of marker expression for the indicated NL or AD samples. Each dot represents a single cell (mean ± SEM; γH2AX: n = 621 for NL, n = 642 for AD; HP1γ: n = 200 for NL, n = 221 for AD, *p = 0.0162; H3K9me3: n = 243 for NL, n = 93 for AD; ns, not significant)