FIGURE 1.

Schematic of study design. Mice in the chronic intermittent ethanol (CIE) only and AIR only groups were nonmanipulated prior to being exposed to 1‐week cycles of ethanol (EtOH) vapor or AIR chambers, with no manipulation during the intervening weeks. Mice in the drinking (DRK) conditions underwent a 2‐week fading procedure followed by 4 weeks of limited‐access ethanol drinking to establish a baseline prior to ethanol vapor (CIE + DRK) or AIR (AIR + DRK) exposure. Cycles of EtOH or AIR exposure were alternated with 5‐day ethanol intake tests. Mice were sacrificed at 2, 10, 26, and 74 hours or 7 days after final exposure to either EtOH or AIR chambers. Among mice in the DRK groups sacrificed at the 7‐day time point, half were allowed to continue limited‐access drinking during test 5, whereas the other half remained abstinent (see Figure 10 for schematic of these groups)