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. 2020 Oct 22;21(Suppl 1):165. doi: 10.1186/s12881-020-01106-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Measuring the kinetics of TBP binding to TATA-containing ODN N2 identical to the HBB promoter in healthy people. Legend: a Dependences of reaction rates on ODN N2 concentrations; b electropherograms from which these curves were derived. TBP concentration was 0.4 nM in all the experiments; the concentrations of a TATA-containing ODN that we used are indicated. TBP–TATA-associated isotherms and k_a and k_d values were inferred from the electropherograms using GraphPad Prism 5 software