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. 2020 Oct 22;21(Suppl 1):165. doi: 10.1186/s12881-020-01106-x

Table 3.

Kinetic and thermodynamic characteristics of TBP–TATA interactions

Gene ODN Alleles:
WT or mut		 Allele Sequences (5′ – 3′ strands),
26 bp		 Prediction Experiments
-lnKD δ -lnKD δ KD, nM ka, M− 1 s− 1 kd, s− 1 t1/2, min
HbB

1

2

WT

rs34500389:T

 -32c > T

agggctgggcataaaagtcagggcag

agggctgggTataaaagtcagggcag

19.20

20.18

 + 0.98

16.81

17.26

 + 0.45

50 ± 7

32 ± 3

(1.4 ± 0.1)*104

2.1 ± 0.1*104

(7.1 ± 0.7)*10−4

(6.6 ± 0,5)*10− 4

16 ± 2

17 ± 2
HbZ

3

4

WT

rs113180943:A

 -29 t > A

agctccctgtatataaggggaccctg

agctccctgtaAataaggggaccctg

20.76

19.28

 −1.48

18.93

17.03

 −1.90

6 ± 1

40 ± 20

(4.5 ± 0.4) *104

(2.3 ± 0,2)*103

3.0 ± 0.6)*10−4

(1.0 ± 0.4)*10− 4

38 ± 4

115 ± 15
EPOR 5 WT cacgtcatctatttttgtctgctacg 18.24 15.59 170 ± 30 (2.8 ± 0,3)*103 (4.6 ± 0.6)*10−4 25 ± 3
6 rs1006576690:A -27 t > A cacgtcatctatAtttgtctgctacg 19.39 + 1.15 18.33 + 2.74 11 ± 3 (9.3 ± 0,7)*103 (1.0 ± 0.3)*10−4 115 ± 15
7 rs971717705:G -29a > G cacgtcatctGtttttgtctgctacg 18.11 −0.13 15.71 + 0.12 150 ± 30 (2.4 ± 0,3)*103 (3.6 ± 0.5)*10−4 32 ± 4
8 rs567946217:A -31c > A cacgtacatAttttttgtctgctacg 19.33 + 1.09 17.68 + 2.09 21 ± 6 (6.8 ± 0.7)*103 (1.4 ± 0.4)*10−4 82 ± 6
9 rs567946217:G -31c > G cacgtсatGtatttttgtctgctacg 18.51 + 0.27 16.34 + 0.75 80 ± 10 (3.6 ± 0.3)*103 (3.0 ± 0.5)*10−4 38 ± 4

ODN Oligodeoxyribonucleotide; WT Ancestral allele; mut Minor allele; KD Equilibrium dissociation constant, which is presented as mean ± standard deviation; δ, the difference between the affinity of TBP for an ODN with and without the SNP in its TATA box expressed as natural logarithms, δ = −ln (KD,Mut) – [−ln (KD,WT)]; ka and kd, association and dissociation rate constants, respectively; t1/2 = ln2/kd, the average half-life of the TBP/ODN complexes; HBB β-globin gene; HBZ Zeta-globin gene; and EPOR Erythropoietin receptor gene