Fig. 4.

Erivation of CM parasite lines. a Parasite lines were derived by limited dilution cloning from 5 Ret+ cases and 3 Ret− cases. After approximately 30 or more cycles of growth and expansion, the parasite lines underwent MSP2 genotyping and var transcriptional profiling at the same time as the parasite-HBMVEC co-culture experiments. b Frequencies of distinct var DBLα tags expressed in the CM derived parasite lines at the time of co-culture experiments. The DBLα tags were subclassified based on BLAST identity to previously annotated full-length var genes and by the number of cysteine residues in the DBLα tag region (see Additional file 6)