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. 2020 Oct 22;10:30. doi: 10.1186/s13395-020-00249-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Time course of hindlimb contractile function following consecutive eccentric challenges. a Longitudinal tetanic force produced by the hindlimbs beginning at week 10 of treatment and including repeat bouts of eccentric challenge (arrows) and recovery. b High-resolution traces of tetani immediately before and 24 h after the first eccentric challenge. c Three-week longitudinal hindlimb force production normalized to pre-challenge levels after the first and (d) second successive eccentric challenges. N = 9-12 mice per group. Mice are MDX unless otherwise noted. Error bars represent SEM. Significance was determined by repeated measure two-way ANOVA with Tukey’s post hoc test (*p < 0.05, ***p < 0.001, ****p < 0.0001; ns, not significant compared to MDX vehicle controls)