Fig. 3.

Representation of the species richness, diversity, and coverage for the simulated bacterial dataset either analyzed by the ASV method (red) or OTU method (blue). Datasets are simulated from the SILVA 16S rRNA gene database with an original community richness varying between 100 (light colored) and 2500 (dark colored). Top panels, Shannon diversity index per original sample richness; middle panels, community richness with increasing sequencing depth; the bottom panels, coverage of each method per taxonomic level