Table 2.
LTQ Orbitrap Velos Pro Acquisition Method
| Parameter | Value |
|---|---|
| Polarity | Positive |
| Full MS Scans | |
| Mass analyser | Orbitrap |
| Orbitrap resolution | 60,000 |
| Scan range (m/z) | 350–2,000 |
| Automatic Gain Control (AGC) target | 1 × 106 |
| Microscans | 1 |
| Dependent MS/MS Scans | |
| Number of dependent scans | 20 (from most intense precursors) |
| Mass analyzer | Ion trap |
| Scan rate | Normal |
| Mass range | Normal |
| Automatic Gain Control (AGC) target | 1 × 104 |
| Microscans | 1 |
| Activation type | Collision-induced dissociation |
| Normalized collision energy (%) | 35 |
| Charge exclusion | Exclude +1 charge state |
| Dynamic exclusion | 60 s |