Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Neural Differentiation Medium
| DMEM/F-12∗ | |
|---|---|
| Neurobasal medium∗ | |
| N2 supplement | 0.5% (v/v) |
| B27 supplement | 1% (v/v) |
| MEM-NEAA | 0.5% (v/v) |
| Glutamax supplement | 1% (v/v) |
| Penicillin/Streptomycin | 1% (v/v) |
| Insulin | 0.025% (v/v) |
| β-Mercaptoethanol | 50 μM |
| BDNF | 20 ng/mL |
| Ascorbic acid | 200 μM |
| Optional: FGF2 | 20 ng/mL (See Troubleshooting step 4) |
Store up to 2 weeks at 4°C.
DMEM/F-12 and neurobasal medium are mixed at 1:1 ratio.
