REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TCF7L2	Cell Signaling	Cat# 2569; RRID:AB_2199816
Chemicals, Peptides, and Recombinant Proteins
mTeSR1	Stem Cell Technologies	Cat# 05875
DMEM-F12	Life Technologies	Cat# 11330057
Neurobasal Media	Life Technologies	Cat# 2110349
FBS	Life Technologies	Cat# 10437028
Amino acids, non-essential	Life Technologies	Cat# 11140050
Penicillin/Streptomycin	Life Technologies	Cat# 15140-122
Glutamax	Life Technologies	Ca# 35050
Insulin	Sigma	Ca# I9278
β-Mercaptoethanol	Sigma	Ca# M7522
N2	Life Technologies	Cat# 17502-048
B27	Life Technologies	Cat# 17504-044
B27 supplement without vitamin A	Life Technologies	Cat# 12587010
bFGF	Millipore	Cat# GF003AF
KnockOut Serum Replacement	Life Technologies	Cat# 10828-028
Matrigel	BD	Cat# 354230
Y-27632	Stem Cell Technologies	Cat# 72304
Dispase (100 mL)	Stem Cell Technologies	Cat# 07913
Accutase (100 mL)	Stem Cell Technologies	Cat# AT104
LDN-193189	Sigma	Cat# SML0559
SB431542	Abcam	Cat# ab120163
XAV939	Sigma	Cat# X3004
BMP7	GIBCO	Cat# PHC9544
PD0325901	Axon Medchem	Cat# Axon 1408
BDNF	Prepotech	Cat# 450-02
Ascorbic acid	Sigma	Cat# A92902
Experimental Models: Cell Lines
HES-3 NKX2-1GFP/w	Elefanty lab	https://www.ncbi.nlm.nih.gov/pubmed/21425409
Other
U-bottom ultra-low-attachment 96-well plate	Corning	CLS7007-24EA
Ultra-low-attachment 24-well plate	Corning	CLS3473
Ultra-low-attachment 6-well plate	Corning	CLS3471-24EA
Orbital shaker	IKA	KS260