Figure 2.

Representative Fluorescence Activated Cell-Sorting Data

(A) No stain control sample. FSC-A and SSC-A are used to initially gate cells (Gate 1, red outline). Cells are further refined based on SSC-H vs. SSC-W (Gate 2, red outline) and subsequently FSC-H vs. FSC-W (Gate 3, red outline). Baseline fluorescence of cells (no stain) in live/dead Violet-A channel vs SSC-A is used to select cells for stained sample (No stain, black outline).

(B) Live/dead stained sample. Same gating strategy as outlined for (A). Note: no stain and Gate 4 (live cells) are the same but appearance is affected by the automatic scaling of the X-axis. This representative sample is from mice at 5 days post injury.

SSC, side scatter; FSC, forward scatter (-A, area; -H, height; -W, width).