Figure 4.

Examination of Potential Batch Effects

(A) Principal component analysis and embedding of all cells evaluated by scRNA-seq from six regeneration stages and non-injured muscle, colored by batch.

(B) UMAP embedding of cells colored by batch.

(C) UMAP embedding of cells colored by regeneration timepoints.

(D) UMAP embedding of cells, colored by cluster. The results suggest that there is no obvious separation based on batch. Batch 1, 0.5 and 3.5 DPI; Batch 2, 10 and 21 DPI; Batch 3, NI; Batch 4, 2 and 5 DPI.

DPI, days post injury; NI, non-injured.