REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
10% Tween 20	Bio-Rad	1662404
50% glycerol	Ricca Chemical Company	3290-32
Bovine Serum Albumin	Gemini Bio	700-105P
Buffer EB	Qiagen	19086
Cardiotoxin (CTX)	Sigma	11061-96-4
Collagenase II	Worthington	LS004177
Dispase	Roche	04942078001
F10 medium	Gibco	11550-043
Fetal bovine serum	HyClone	SH30088
Ketamine HCl	Akron	59399-114-10
Low TE buffer	Thermo Fisher Scientific	12090-015
Penicillin-Streptomycin	ThermoFisher	15070063
Phosphate-buffered saline	Gibco	21600-069
Red blood cell lysis solution	Promega	Z3141
Xylazine	Akron	59399-110-20
Critical Commercial Assays
Chromium Single Cell 3′ GEM Library & Gel Kit v3	10x Genomics	PN-1000075
Chromium Single Cell B Chip Kit	10x Genomics	PN-1000153
Chromium i7 Multiplex Kit	10x Genomics	PN-120262
SPRI-select Reagent Kit	Beckman Coulter	B23318
Zombie Violet Fixable Viability Dye	BioLegend	423113
Deposited Data
scRNA-seq regenerating muscle data	GEO	GSE138826
Experimental Models: Organisms/Strains
C5Bl6/ WT mice	Purdue TG Core	N/A
Software and Algorithms
Cell Ranger Software	10x Genomics	v3.1
Seurat	Satija lab: satijalab.org/seurat/	v3
Other
10 mL syringe	BD Falcon	302995
10x Magnetic Separator	10x Genomics	230003
18G1 1/2 needles	BD	305196
40 μm filters	BD Falcon	352340
27-gauge insulin syringes	Exel Int	26028
PCR tubes, 0.2 mL 8-tube strips, sterile	Eppendorf	951010022
Syringe filter	CellTreat	229751
2 mL low binding conical-bottom microcentrifuge tube, nuclease free	VWR	16466-042
Centrifuge	ThermoFisher	IEC Centra CL2
15 mL conical tube	Fisher Scientific	14-959-53A
PCR machine	N/A	N/A
Vortex genie	VWR	SI-0236 120V
Normal saline solution	Vedco	50989-885-17
Low retention 10 μL filtered pipette tips	Fisher Scientific	02-707-000
Low retention 200 μL filtered pipette tips	Fisher Scientific	02-707-006
Low retention 1,000 μL filtered pipette tips	Fisher Scientific	02-707-008