Complete Neurobasal (CNB) Media
| Reagent | Final Concentration | Volume (mL) |
|---|---|---|
| Neurobasal Medium | 1× | 472.5 |
| B-27 Supplement | 2% | 10 |
| Sodium pyruvate | 1 mM | 5 |
| GlutaMax | 1× | 5 |
| Penicillin Streptomycin | 50,000 U/mL | 5 |
| Fetal Bovine Serum | 0.5% | 2.5 |
| Total | 500 |
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