Reagent | Final Concentration | Volume (μL) |
---|---|---|
HBSS | 1× | 4,910 |
Papain | 14 U/mL | 80 |
0.5M EDTA | 0.5 μM | 5 |
1M CaCl2 | 1 μM | 5 |
Total | 5,000 |
Reagent | Final Concentration | Volume (μL) |
---|---|---|
HBSS | 1× | 4,910 |
Papain | 14 U/mL | 80 |
0.5M EDTA | 0.5 μM | 5 |
1M CaCl2 | 1 μM | 5 |
Total | 5,000 |