Fixing Solution
| Reagent | Final Concentration | Amount |
|---|---|---|
| 37% formaldehyde | 3.7% | 50 mL |
| 10× PBS | 1× | 50 mL |
| Sucrose | 4% | 20 g |
| Deionized water | n/a | 400 mL |
| Total | 500 mL |
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