REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bassoon	Enzo life Sciences	Cat # SAP7F407; RRID: AB_2313990
Cy3-conjugated donkey anti-mouse	Jackson ImmunoResearch laboratories	Cat #715-165-150; RRID: AB_2340813
FITC-conjugated donkey anti-goat	Jackson ImmunoResearch laboratories	Cat #705-095-147; RRID: AB_2340401
EGFP	Rockland	Cat # 600-101-215; RRID: AB_218182
Chemicals, Peptides, and Recombinant Proteins
Neurobasal medium	ThermoFisher Scientific	Cat #21103049
B-27 supplement (50×)	ThermoFisher Scientific	Cat #17504-044
Penicillin/streptomycin	ThermoFisher Scientific	Cat #15140122
HBSS (Hank’s Balanced Salt Solution)	ThermoFisher Scientific	Cat #14065056
Sodium pyruvate	ThermoFisher Scientific	Cat #11360070
GlutaMax supplement	ThermoFisher Scientific	Cat #35050061
Minimal essential medium (MEM)	Welgene	LM007-12
Poly-D-lysine hydrobromide	Sigma-Aldrich	P0899
37% formaldehyde	Biosesang	F1012
Bovine serum albumin	Sigma-Aldrich	A7906
Horse serum	Gibco	16050122
EDTA	Affymetrix	15701
Calcium chloride	Affymetrix	12531
Magnesium chloride	Sigma-Aldrich	M8266
Papain	Worthington	LS003126
Hydrochloric acid	Reagents Duksan	Cat #1129
Sodium hydroxide	DAEJUNG	7572-3700
Sucrose	Sigma-Aldrich	S7903
Triton X-100	Sigma-Aldrich	T8787
Nitric acid	Reagents Duksan	Cat #1538
Boric acid	Sigma-Aldrich	B6768
Critical Commercial Assays
NucleoBond ® Xtra Midi kit for transfection-grade plasmid DNA	MACHEREY-NAGEL	MN740410
CalPhos Kit for neuron transfections	Takara	Cat #631312
Experimental Models: Organisms/Strains
Mice: E17 pregnant females (C57BL/6J)	The Jackson Laboratory	000664
Rat: E18 pregnant females (Sprague-Dawley)	Daehan Biolink	N/A
Recombinant DNA
pCAGG-VGLUT1-Venus	Han et al., 2020a	N/A
Software and Algorithms
MetaMorph	Molecular Devices	https://www.moleculardevices.com
Photoshop	Adobe	https://www.adobe.com
Microsoft Excel	Microsoft	https://www.microsoft.com
Zen 2.6	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html
Other
Surgical scissors	Fine Science Tools	Cat #14054-13
Student Dumont #5 forceps	Fine Science Tools	Cat #91150-20
Dressing forceps	KASCO	Cat #6-004
Dry ice	N/A	N/A
CO2 incubator	ESCO	Cat # CCL-170B-8-FD
Centrifuge	Labogene	https://labogene.co.kr
Cover glasses	Marienfeld	Cat #0111580
0.2-μm syringe filters	Sartorius Stedim Biotech	https://www.sartorius.com
Dissecting microscope	Nikon	https://www.microscope.healthcare.nikon.com
Dissection hood	Bio-free	https://biofree.co.kr
Conical tubes	Neurex	https://saehanlab.co.kr
Microcentrifuge tubes	Axygen	https://www.corning.com
12-well plates, 6-well plates	SPL	https://www.spllifesciences.com
Furnace	JEIO Tech	Cat #MF-G
Petri dishes	SPL	https://www.spllifesciences.com
Confocal microscope (LSM800)	Zeiss	http://www.zeiss.com