REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
DMEM:F12 medium | Gibco/ThermoFisher | Cat# 11330032 |
FBS | Sigma-Aldrich | Cat# F4135 |
ITS; Insulin-Transferrin-Selenium (100×) | Gibco/ThermoFisher | Cat# 41400045 |
Penicillin-Streptomycin (10,000 U/mL) (100×) | Gibco/ThermoFisher | Cat# 15140163 |
Dexamethasone-Water Soluble | Sigma-Aldrich | Cat# D2915 |
Hydrogen peroxide 30% stabilized, AnalaR NORMAPUR® | VWR chemicals Singapore | Cat# VWRC23619.297 |
Phosphate buffered saline (10×) | Sigma-Aldrich | Cat# P5493 |
TrypLE™ Express Enzyme (1×), phenol red | Gibco/ThermoFisher | Cat# 12605028 |
Hoechst 33342 | Abcam | Cat# ab228551 |
Alexa Fluor 488 antibody | Molecular Probes, ThermoFisher | Cat# A-11094 |
γH2A.X antibody | Abcam /ThermoFisher | Cat# ab81299 |
Critical Commercial Assays | ||
Cellular Senescence Assay kit | Merck Millipore | Cat# KAA002 |
Experimental Models: Cell Lines | ||
AML12 cells | ATCC | ATCC® CRL-2254™ |
Software and Algorithms | ||
PRISM 8.0 | Graphpad | Version 8.0 |
Other | ||
Biological Safety Cabinet | Esco Micro Pte. Ltd. | Model abculture® Class II, Type A2 (E-Series) |
Incubator | NuAire | Model No NU-5500E |
Water bath | Julabo | Model No TW12 |
Inverted microscope | Nikon Eclipse TS100 | N/A |
Benchtop Refrigerated Centrifuge | Eppendorf/VWR Singapore Pte Ltd | Cat# EPPE5811000.320 Model 5810 R |
Countess II automated Cell counter | ThermoFisher | Cat# AMQAX1000 |
Nunc™ Cell Culture Treated Flasks with Filter Caps (T175 and T25) | ThermoFisher | Cat# 178885 (T175) Cat# 136196 (T25) |
Falcon™ 15 mL Conical Centrifuge Tubes | Fisher Scientific | Cat# 14-959-49B |