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. 2020 Jul 1;1(2):100064. doi: 10.1016/j.xpro.2020.100064
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

DMEM:F12 medium Gibco/ThermoFisher Cat# 11330032
FBS Sigma-Aldrich Cat# F4135
ITS; Insulin-Transferrin-Selenium (100×) Gibco/ThermoFisher Cat# 41400045
Penicillin-Streptomycin (10,000 U/mL) (100×) Gibco/ThermoFisher Cat# 15140163
Dexamethasone-Water Soluble Sigma-Aldrich Cat# D2915
Hydrogen peroxide 30% stabilized, AnalaR NORMAPUR® VWR chemicals Singapore Cat# VWRC23619.297
Phosphate buffered saline (10×) Sigma-Aldrich Cat# P5493
TrypLE™ Express Enzyme (1×), phenol red Gibco/ThermoFisher Cat# 12605028
Hoechst 33342 Abcam Cat# ab228551
Alexa Fluor 488 antibody Molecular Probes, ThermoFisher Cat# A-11094
γH2A.X antibody Abcam /ThermoFisher Cat# ab81299

Critical Commercial Assays

Cellular Senescence Assay kit Merck Millipore Cat# KAA002

Experimental Models: Cell Lines

AML12 cells ATCC ATCC® CRL-2254™

Software and Algorithms

PRISM 8.0 Graphpad Version 8.0

Other

Biological Safety Cabinet Esco Micro Pte. Ltd. Model abculture® Class II, Type A2 (E-Series)
Incubator NuAire Model No NU-5500E
Water bath Julabo Model No TW12
Inverted microscope Nikon Eclipse TS100 N/A
Benchtop Refrigerated Centrifuge Eppendorf/VWR Singapore Pte Ltd Cat# EPPE5811000.320
Model 5810 R
Countess II automated Cell counter ThermoFisher Cat# AMQAX1000
Nunc™ Cell Culture Treated Flasks with Filter Caps (T175 and T25) ThermoFisher Cat# 178885 (T175)
Cat# 136196 (T25)
Falcon™ 15 mL Conical Centrifuge Tubes Fisher Scientific Cat# 14-959-49B