Figure 3.
Gating Strategy for Myeloid Cells in Brain, Epidermis and Liver
(A) Gating strategy of brain microglia (CD45intCD11b+F4/80+Ly6C−) of 8-week-old mice.
(B) Gating strategy of epidermal Langerhans cells (LCs) (CD45+Ly6G−Siglec F−CD11b+F4/80+) of 8-week-old mice.
(C) Gating strategy of liver Kupffer cells (KCs) (CD45+Lin−CD11b+F4/80+Tim-4+) of 8-week-old mice. Lineage markers include CD3e, CD19, CD49b and Ly6G.