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Hepatocyte Purification
(A) Cells from the dissected liver are released into suspension and centrifuged. The pellet contains hepatocytes and the rest of the cells are left in the supernatant.
(B) The hepatocyte pellet (A) is re-suspended with Percoll solution and centrifuged again. The pellet contains viable hepatocytes while dead cells and debris are left in the supernatant.
