Perfusion Buffer
| Reagent | Final Concentration | Stock Concentration | Volume (mL) |
|---|---|---|---|
| HBSS no Ca2+ no Mg2+ no phenol red | - | - | 487 |
| EDTA | 0.5 mM | 0.5 M | 0.5 |
| HEPES | 25 mM | 1 M | 12.5 |
| Total | 500 |
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