REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
HBSS with calcium, magnesium and phenol red | Biological industries | 02-015-1A |
HBSS no calcium, no magnesium and no phenol red | Biological industries | 02-018-1A |
EDTA (0.5 M) | Fisher bioreagents | BP2482-500 |
HEPES (1 M) | Sigma-Aldrich | H0887-100ML |
Ketamine (Clorketam) | Vetoquinol | 00 92297 43 082 |
Xylazine (Sedaxylan) | EuroVet | SEDAXYLAN |
DMEM low glucose | Biological industries | 01-050-1A |
Dulbecco's Phosphate Buffered Saline (DPBS) without calcium and magnesium | Biological industries | 02-023-1A |
Phosphate Buffered Saline (PBS) ×10 | Hylabs | BP507/500D |
L-Glutamine Solution | Biological industries | 03-020-1B |
Penicillin-Streptomycin Solution | Biological industries | 03-031-1B |
William's E Medium, no glutamine | Gibco | 12551-032 |
Fetal Bovine Serum (FBS) | Biological industries | 04-007-1A |
Collagen | Sigma-Aldrich | C3867-1VL |
Percoll | Santa Cruz biotechnologies | sc-500790A |
Trypan Blue Solution | Biological industries | 03-102-1B |
Liberase™ TM Research Grade | Sigma-Aldrich | 05401127001 |
Experimental Models: Organisms/Strains | ||
Mouse strain: C57BL/6JOlaHsd | Envigo | N/A |
Other | ||
Insulin syringe | Becton Dickinson (BD) | BD 324912 |
Cell strainer, 70 μm | Corning | CLS431751-50EA |
Cell lifter | Corning | CLS3008 |
Peristaltic pump | Gilson | Miniplus 3 PVC tubing 2.06 mm diameter |
Mouse dissection tray | N/A | N/A |
Water bath | N/A | N/A |
Sterile 50 mL centrifuge tubes | Corning | 430829 |
Sterile 25 mL serological pipettes | Bio-SORFA | 315100 |
27 gauge needle | BD Microlance | 302200 |
70% ethanol | N/A | N/A |
Head wearing magnifier eye loupe (optional) | N/A | N/A |