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. 2020 Aug 13;1(2):100086. doi: 10.1016/j.xpro.2020.100086
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

HBSS with calcium, magnesium and phenol red Biological industries 02-015-1A
HBSS no calcium, no magnesium and no phenol red Biological industries 02-018-1A
EDTA (0.5 M) Fisher bioreagents BP2482-500
HEPES (1 M) Sigma-Aldrich H0887-100ML
Ketamine (Clorketam) Vetoquinol 00 92297 43 082
Xylazine (Sedaxylan) EuroVet SEDAXYLAN
DMEM low glucose Biological industries 01-050-1A
Dulbecco's Phosphate Buffered Saline (DPBS) without calcium and magnesium Biological industries 02-023-1A
Phosphate Buffered Saline (PBS) ×10 Hylabs BP507/500D
L-Glutamine Solution Biological industries 03-020-1B
Penicillin-Streptomycin Solution Biological industries 03-031-1B
William's E Medium, no glutamine Gibco 12551-032
Fetal Bovine Serum (FBS) Biological industries 04-007-1A
Collagen Sigma-Aldrich C3867-1VL
Percoll Santa Cruz biotechnologies sc-500790A
Trypan Blue Solution Biological industries 03-102-1B
Liberase™ TM Research Grade Sigma-Aldrich 05401127001

Experimental Models: Organisms/Strains

Mouse strain: C57BL/6JOlaHsd Envigo N/A

Other

Insulin syringe Becton Dickinson (BD) BD 324912
Cell strainer, 70 μm Corning CLS431751-50EA
Cell lifter Corning CLS3008
Peristaltic pump Gilson Miniplus 3
PVC tubing 2.06 mm diameter
Mouse dissection tray N/A N/A
Water bath N/A N/A
Sterile 50 mL centrifuge tubes Corning 430829
Sterile 25 mL serological pipettes Bio-SORFA 315100
27 gauge needle BD Microlance 302200
70% ethanol N/A N/A
Head wearing magnifier eye loupe (optional) N/A N/A