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Illustrative Overview of the Urinary EV Separation Protocol
After collection and preparation of the urine sample, pre-enrichment of urinary EV is performed by ultrafiltration. Urinary EV are separated and recovered from this ultrafiltrate by the orthogonal implementation of a bottom-up density gradient and size-exclusion chromatography. The EV sample is concentrated to a volume suitable for downstream EV characterization and integrative omics analysis.
