Density Gradient Buffer 2
| Reagent | Final Concentration | Add to 1L |
|---|---|---|
| Sucrose | 0.25 M | 85.58 g |
| EDTA | 1 mM | 0.29 g |
| Tris base | 10 mM | 1.21 g |
| HCl 5 M | n/a | ~1.05 mL |
| Ultrapure water | n/a | Up to 1 L |
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