REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alix	Cell Signaling Technology	Cat#2171S
TSG-101	Santa Cruz Biotechnology	Cat#sc-7964
Flotillin-1	BD Biosciences	Cat#610820
Syntenin-1	Abcam	Cat#ab133267
CD9	Cell Signaling Technology	Cat#D3H4P
PODXL	Abcam	Cat#ab150358
AQP2	ThermoFisher Scientific	Cat#PA5-38004
UPK1B	Sigma-Aldrich	Cat#HPA031800
PSA/KLK3	Cell Signaling Technology	Cat#D11E1
GM130	BD Biosciences	Cat#610822
THP	Santa Cruz Biotechnology	Cat#sc-20631
PMP70	Sigma-Aldrich	Cat#P0497
Sheep anti-mouse HRP-linked Ab	GE Healthcare life sciences	Cat#NA931V
Donkey anti-rabbit HRP-linked Ab	GE Healthcare life sciences	Cat#NA934V
Biological Samples
Urine	Human	Ethical Committee of Ghent University Hospital approval EC/2015/0260
Chemicals, Peptides, and Recombinant Proteins
OptiPrep (60% w/v) iodixanol	Axis-Shield	Cat#AXS-1114542
Sucrose	Sigma-Aldrich	Cat#S0389
EDTA (Titriplex III)	Merck Millipore	Cat#1084180100
Tris base	Sigma-Aldrich	Cat#T6066
HCl (standardized solution 5.0 N)	VWR	Cat#35638.K2
Ultrapure water	Invitrogen	Cat#AM9932
PBS (pH 7.2)	ThermoFisher Scientific	Cat#20012019
Sepharose CL-2B	GE Healthcare	Cat#17014001
Critical Commercial Assays
Multistix 10SG Urinalysis Reagent Test Strips (Siemens Healthineers)	VWR	Cat#10789-338
Deposited Data
EV-TRACK	(Van Deun et al., 2017)	EV-TRACK ID: EV190064
ProteomeXchange	(Vizcaíno et al., 2014)	Dataset identifier: PXD015289
GEO	(Edgar, 2002)	Dataset identifier: GSE131689
Other
100 mL urine collection container with screw cap (sterile)	Sarstedt	Cat#75.562.105
1.5 mL Eppendorf Safe-Lock microcentrifuge tubes (sterile)	Sigma-Aldrich	Cat#EP022363212
5 mL Eppendorf tubes (sterile)	Sigma-Aldrich	Cat#EP0030119460
17 mL Thinwall polypropylene tube	Beckman Coulter	Cat#337986
Conical 50 mL polypropylene tubes (sterile)	Greiner Bio One	Cat#210261
Centricon Plus-70 centrifugal filter units (10 kDa MWCO)	Merck Millipore	Cat#UFC701008
Amicon Ultra-2 centrifugal filter units (10 kDa MWCO)	Merck Millipore	Cat#UFC201024
10 mL Disposable syringe (sterile)	Romed	Cat#3SYR-10ML
Combi-Stopper closing cones	B Braun	Cat#4495101
Parafilm M laboratory film	Bemis Company	Cat#PM-996
Nylon-net filters (20.0 μm pore size)	Merck Millipore	Cat#NY2002500
Bottle-top vacuum filters (0.22 μm pore size)	Sigma-Aldrich	Cat#CLS430624
Optima XPN-80 Ultracentrifuge	Beckman Coulter	Cat#A99839
SW 32.1 Ti Swinging-Bucket Rotor and SW 32 Ti Rotor Bucket Set	Beckman Coulter	Cat#369651
Biomek 4000 Laboratory Automation Workstation	Beckman Coulter	Cat#A99749
Magnetic stirrer	N/A	N/A
P100 - P5000 Manual pipettes	N/A	N/A
P100 - P5000 Pipette tips (sterile)	N/A	N/A
Electronic pipettor	BrandTech Scientific	Cat#26330
5 mL and 25 mL Serological pipettes	nerbe plus	Cat#12-441-9105 Cat#12-481-9102
Cooled benchtop centrifuge with swinging-bucket rotor	Eppendorf	Cat#5811000320
100 mL and 1 L Glass laboratory bottles (sterile)	Duran	Cat#218012417 Cat#218015414
Vacuum pump	N/A	N/A
Laboratory stand with three-prong extension clamps	VWR	Cat#470135-122
Vortex mixer	N/A	N/A
Multi-well microplate reader	Biotek	Cat#SynergyHTX
Nunc MicroWell 96-Well Microplate	VWR	Cat#734-2097