Recipe for 1 L 70:30 Medium for Sporulation Plates (Makes 28–35 mL Plates)
| Reagent | Amount |
|---|---|
| Bacto Peptone | 63 g |
| Proteose Peptone | 3.5 g |
| Ammonium sulfate | 0.7 g |
| Tris base | 1.06 g |
| BHI broth base | 11.1 g |
| Yeast extract | 1.5 g |
| Agar | 15 g |
| L-cysteine 10% w/v | 3 mL |
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