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PCR Mix (including 10% Extra)
| Reagent | Final Concentration | Volume (μL) |
|---|---|---|
| H2O | n/a | 11.3 μl |
| 5x Q5 buffer | n/a | 5.5 μl |
| 5x GC-Enhancer | n/a | 5.5 μl |
| dNTPs | 10 mM each | 2.8 μl |
| PrimerMix (see above) | PCR 1: 0.4 μM:0.2μM:0.2μM; PCR 2: 0.2μM:0.2μM | 1.1 μl |
| Template | 100 ng/μl | 1 μl |
| Polymerase | 5.5 units | 0.3 μl |
