| 1 | 98°C | 4 min |
| 2 | 98°C | 30 s |
| 3 | 68°C | 30 s (−1°C per cycle) |
| 4 | 72°C | 6 min, back to step 2 (5x) |
| 5 | 98°C | 30 s |
| 6 | 63°C | 30 s |
| 7 | 72°C | 6 min, back to step 5 (40x) |
| 8 | 72°C | 5 min |
| 1 | 98°C | 4 min |
| 2 | 98°C | 30 s |
| 3 | 68°C | 30 s (−1°C per cycle) |
| 4 | 72°C | 6 min, back to step 2 (5x) |
| 5 | 98°C | 30 s |
| 6 | 63°C | 30 s |
| 7 | 72°C | 6 min, back to step 5 (40x) |
| 8 | 72°C | 5 min |