| 1 | 98°C | 4 min |
| 2 | 98°C | 30 s |
| 3 | 68°C | 30 s (−1°C per cycle) |
| 4 | 72°C | 6 min, back to step 2 (5x) |
| 5 | 98°C | 30 s |
| 6 | 63°C | 30 s |
| 7 | 72°C | 6 min, back to step 5 (40x) |
| 8 | 72°C | 5 min |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.