REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor 488 Goat anti-Mouse	ThermoFisher	Cat#A11001
Alexa Fluor 488 Goat anti-Rabbit	ThermoFisher	Cat#A11008
Alexa Fluor 555 Goat anti-Mouse	ThermoFisher	Cat#A21429
Alexa Fluor 555 Goat anti-Rabbit	ThermoFisher	Cat#A21424
Anti-FOXG1, rabbit	Abcam	Cat#18259
Anti-GABA, rabbit	Sigma	Cat#A-2052
Anti-TUBB3, mouse	Biolegend	Cat#801202
Anti-TUBB3, rabbit	Biolegend	Cat#802001
Chemicals, Peptides, and Recombinant Proteins
Accutase	Gibco	Cat#A11105-01
Ara-C	Merck	Cat#C1768
B-27 supplement (50x)	Gibco	Cat#12587-010
DAPT	Sigma	Cat#D5942-5MG
DMEM/F-12	Gibco	Cat#11320-074
DMSO	Sigma	Cat#D4540
Doxycycline	Sigma	Cat#D9891-1G,
dTTP	VWR Life Science	Cat#20-1041
dCTP	VWR Life Science	Cat#20-1020-88
dATP	VWR Life Science	Cat #20-1011
dGTP	VWR Life Science	Cat #20-1030-88
EDTA	Sigma	Cat#E6635
Geltrex	Gibco	Cat#A14132-02
GlutaMAX (100x)	Gibco	Cat#35050-38
KnockOut Serum Replacement	Gibco	Cat#10828-028
Laminin	Sigma	Cat#L2020
L755507	Sigma Aldrich	Cat#SML 1362
N2 Supplement (100x)	GE Healthcare	Cat#T11292005
Neurobasal	Gibco	Cat#21103-049
DPBS (1x)	Gibco	Cat#14190-094
Poly-L-ornithine hydrobromide	Sigma Aldrich	Cat#P3655
Recombinant human BDNF	Cell GS	Cat#GFH1
ROCK Inhibitor	Cellagen Technology LLC	Cat#C9127-2
StemMACS iPS-Brew 50x supplement	Miltenyi Biotec	Cat#130-107-086
StemMACS iPS-Brew	Miltenyi Biotec	Cat#130-107-087
Trehalose	Sigma	Cat#T9531
Q5 Buffer	New England BioLabs	Cat#B90275
Q5 High-Fidelity DNA Polymerase	New England BioLabs	Cat#M0492L
Critical Commercial Assays
Amaxa nucleofection kit V	Lonza	Cat#VVSA-1003
Experimental Models: Cell Lines
C-14 m-s11 NGN2 iPSCs (for generation of iGlutNs)	Rhee et al., 2019 Meijer et al., 2019	n/a
C-133bm-s4 ADP#8 iPSCs (for generation of iGABANs)	Rhee et al., 2019 Meijer et al., 2019	n/a
Oligonucleotides
AAVS1 insertion validation primer fwd: ACCAACGCCGACGGTATCAG	Integrated DNA Technologies	n/a
AAVS1 insertion primer rv: CAGACCCTTGCCCTGGTGGT	Integrated DNA Technologies	n/a
AAVS1 wt site sequence primer rv: CACCAGGATCAGTGAAACGC	Integrated DNA Technologies	n/a
AAVS1 fwd 3′ primer TACCACCGATTCTATGCCCC	Integrated DNA Technologies	n/a
AAVS1 rv 3′ primer AGGATGCAGGACGAGAAACA	Integrated DNA Technologies	n/a
Recombinant DNA
AAVS1-ASCL1-P2A-DLX2	Rhee et al., 2019	n/a
AAVS1-NGN2	Rhee et al., 2019 Meijer et al., 2019	n/a
AAVS1-TALEN-L	Dr. Su-Chun Zhang (University of Wisconsin) Qian et al., 2014	n/a
AAVS1-TALEN-R	Dr. Su-Chun Zhang (University of Wisconsin) Qian et al., 2014	n/a
Other
40 μm Cell Strainer	Falcon	Cat#352340
Trypan Blue Stain (0.4%)	Gibco	Cat#15250-061
50 mL polypropylene conical tube	Falcon	Cat#352070
Cryotube 1.8 mL	Thermo Scientific	Cat#377267
Counting chamber Fuchs-Rosenthal	Optik Labor	Cat#270000