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. 2020 Jun 3;1(1):100019. doi: 10.1016/j.xpro.2020.100019

Figure 13.

Figure 13

Schematic: from raw data to area under the curve

(A) Raw data (area μm2 per well per time point) can be normalized to time point 0 min. Normalized data depicted in a graph shows relative increase in swelling per well over time (baseline is set to 100%). Here, duplicate wells are measured for swelling at 0.02; 0.128; 0.8 and 5 μM fsk for 60 min.

(B) The AUC of relative increase in swelling is calculated in Excel.

(C) Using AUC calculations for the different conditions, a condensed graph can be created which represents overall relative swelling data per condition over 60 min. Graph C shows the average AUC data of the duplicate wells (from B) with SD.