Table 5.

Experimental Plate Set Up for Measurement of Drug Response of Reference Organoid Cell Lines for Validation Data

(Day 1)	CM +/+		CM +/ + + VX-661 3 μM		CM +/+		CM +/ + + VX-661 3 μM
(Day 2)	Fsk stimuli				Fsk stimuli + VX-770 (3 μM)
Fsk concentration	0.008 μM	5 μM	0.008 μM	5 μM	0.008 μM	5 μM	0.008 μM	5 μM
	0.008 μM	5 μM	0.008 μM	5 μM	0.008 μM	5 μM	0.008 μM	5 μM
	0.02 μM	2 μM	0.02 μM	2 μM	0.02 μM	2 μM	0.02 μM	2 μM
	0.02 μM	2 μM	0.02 μM	2 μM	0.02 μM	2 μM	0.02 μM	2 μM
	0.05 μM	0.8 μM	0.05 μM	0.8 μM	0.05 μM	0.8 μM	0.05 μM	0.8 μM
	0.05 μM	0.8 μM	0.05 μM	0.8 μM	0.05 μM	0.8 μM	0.05 μM	0.8 μM
	0.128 μM	0.32 μM	0.128 μM	0.32 μM	0.128 μM	0.32 μM	0.128 μM	0.32 μM
	0.128 μM	0.32 μM	0.128 μM	0.32 μM	0.128 μM	0.32 μM	0.128 μM	0.32 μM
	Per organoid reference line