REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
Human biliary tract carcinoma tissue samples	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
Advanced DMEM/F12	Thermo Fisher Scientific	Cat# 12634010
GlutaMAX Supplement	Thermo Fisher Scientific	Cat# 35050061
HEPES	Thermo Fisher Scientific	Cat# 15630080
Penicillin-Streptomycin	Thermo Fisher Scientific	Cat# 15140122
B-27 Supplement	Thermo Fisher Scientific	Cat# 17504044
N-2 Supplement	Thermo Fisher Scientific	Cat# 17502048
Nicotinamide	Sigma-Aldrich	Cat# N0636
N-Acetyl-L-cysteine	Sigma-Aldrich	Cat# A9165
[Leu15]-Gastrin I human	Sigma-Aldrich	Cat# G9145
Recombinant Mouse EGF	Thermo Fisher Scientific	Cat# PMG8043
Y-27632	Wako	Cat# 253-00513
Forskolin	Tocris Bioscience	Cat# 1099
A83-01	Tocris Bioscience	Cat# 2939
R-spondin 1 expressing 293T cell line	Merck Millipore	Cat# SCC111
Matrigel (growth factor reduced, phenol red-free)	Corning	Cat# 356231
Dispase II	Thermo Fisher Scientific	Cat# 17105041
Collagenase type XI	Sigma-Aldrich	Cat# C7657
TrypLE Express	Thermo Fisher Scientific	Cat# 12605028
Recovery™ Cell Culture Freezing Medium	Thermo Fisher Scientific	Cat# 12648010