REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli DH5α	WEIDI	CAT# DL1001
A. baumannii ATCC 17978	ATCC	ATCC® 17978
A. baumannii XH386	(Fang et al., 2016)	N/A
Chemicals, Peptides, and Recombinant Proteins
Apramycin sulfate	Sangon	CAT# A600090
Kanamycin sulfate	Sangon	CAT# A600286
Spectinomycin hydrochloride	Meilunbio	CAT# MB1497
Glycerol	Hushi	CAT# 10010618
Sucrose	Sangon	CAT# A502792-0500
LB Broth Powder	Sangon	CAT# A507002-0250
LB Agar Powder	Sangon	CAT# A507003-0250
2×Es Taq MasterMix (Dye)	CoWin Biosciences	CAT# CW0690M
T4 Polynucleotide Kinase	TAKARA	CAT# 2021S
T4 DNA Ligase	NEB	CAT# M0202S
BsaI-HFv2	NEB	CAT# R3733S
HiPure Plasmid Micro Kit	Magen	CAT# P1001-03C
Ezup Column Bacteria Genomic DNA Purification Kit	Sangon	CAT# B518255-0050
Recombinant DNA
pAT04	(Tucker et al., 2014)	N/A
pCasAb-apr	This Paper	Addgene 121998
pSGAb-km	This Paper	Addgene 121999
PSGAb-spe	This Paper	Addgene 122000
PBECAb-apr	This Paper	Addgene 122001
Other
Gene Pulser Xcell™ Electroporation System	Bio-rad	CAT# 165-2660; CAT# 165-2662
2 mm electroporation cuvette	Bio-Rad	CAT# 1652082