Differentiation Medium (250 ml): Store at 4C for Up to 2 Weeks

125 mL DMEM/F12

125 mL Neurobasal

1.25 mL N2 supplement

2.5 mL B27 ± vitamin A supplement (Type I is without Vitamin A; Type II is WITH Vitamin A)

62.5 μL insulin

87.5 μL 2-ME solution (1:100 dilution in DMEM/F12)

2.5 mL Glutamax supplement

1.25 mL MEM-NEAA

2.5 mL P/S