REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
ROCK inhibitor Y-27632 2HCl (50 μM final concentration; Dissolve 5 mg in 2.96 mL water for a 5 mM stock. Dilute stock 1:100 in neural induction medium for final of 50 μM)	Selleck Chemicals	Cat# S1049; N/A
DMEM/F12 Medium	Hyclone	Cat# SH30126.01; N/A
L-Glutamine-200 mM (100X)	Life Technologies	Cat# 25030-149; N/A
Recombinant human EGF 1mg	R&D Systems	Cat# 3718-FB-01M; N/A
Recombinant human bFGF 1mg	R&D Systems	Cat# 236-EG-01M; N/A
N2 supplement	Life Technologies/GIBCO	Cat# 17502-048; N/A
Glutamax	Thermo Fisher Scientific	Cat# 35050-061; N/A
Minimum Essential Medium-Nonessential Amino Acids (MEM-NEAA)	Thermo Fisher Scientific	Cat# 11140-050; N/A
Neurobasal Medium	Life Technologies	Cat# 21103-049; N/A
Neurobasal-A Medium	Life Technologies	Cat# 10888-022; N/A
B27 supplement WITHOUT vitamin A	Life Technologies/GIBCO	Cat# 12587-010; N/A
2-mercaptoethanol	Thermo Fisher Scientific	Cat# 21985-023; N/A
Insulin	Sigma	Cat# I9278-5ml; N/A
Matrigel™ Membrane Matrix for Organoid Droplets	Corning	Cat# 356234; N/A
B27 supplement WITH vitamin A	Life Technologies/GIBCO	Cat# 17504-044; N/A
hESC-Qualified Matrigel™	Corning	Cat# 354277; N/A
Normocin	Invivogen	Cat# Ant-nr-2; N/A
0.5M EDTA pH 8.0	Invitrogen	Cat# 15575-038; N/A
mTeSR1	STEM CELL TECHNOLOGIES	Cat# 05850; N/A
STEMdiff™ Neural Induction Medium	STEM CELL TECHNOLOGIES	Cat# 05835; N/A
STEMdiff™ Neural Rosette Selection Reagent	STEM CELL TECHNOLOGIES	Cat# 05832; N/A
Penicilin-Streptomycin solution (20mL)	Life Technologies/GIBCO	Cat# 15140148; N/A
Experimental Models: Cell Lines
Patient-Derived Glioma Stem Cells: 827, 1228, 211, 923, 308, 0728, 0320, 0517, 0607, 0810, and 1206	National Institutes of Health and Weill Cornell Medicine/NYU Presbyterian Hospital	N/A
NIH-registered human H1 (WA01) embryonic stem cells	WiCell Research Institute	Cat# WA01; RRID: CVCL_9771
NIH-registered human H9 (WA09) embryonic stem cells	WiCell Research Institute	Cat# WA09; RRID: CVCL_9773
Other
Ultra low-attachment 96-well round bottom plates	Corning	Cat# 7007; N/A