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. 2020 Jul 24;1(2):100068. doi: 10.1016/j.xpro.2020.100068

Figure 1.

Figure 1

Effect of Plating Density on Differentiation Efficiency

(A–C) Efficiency of definitive endoderm (DE) derivation at day 5 varies based on iPSC confluency before starting the differentiation. Representative immunofluorescence (IF) images of cells show expression of FOXA2 (green) and SOX17 (red). Nuclei are counterstained with DAPI (blue). Differentiation started with too few cells (<70% confluency) or too many cells results in poor (A) or low (B) DE differentiation. Differentiation started with optimal plating density of 70%–80% confluency at the time of starting the experiment results in best DE differentiation (C). Scale bars, 200 μm.