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Markers for Characterizing Different Stages of Human iPNEC Differentiation
| Cell types | Markers | Assays |
|---|---|---|
| Definitive endoderm (DE) | FOXA2, SOX17 (CXCR4) | Immunofluorescence |
| Anterior foregut endoderm (AFE) | FOXA2, SOX17 | Immunofluorescence |
| Lung progenitors | NKX2.1 (lung endoderm marker), ZO1 (tight junction marker), EPCAM (epithelial marker) | Immunofluorescence |
| Induced PNECs (iPNECs) | SYP, PGP9.5, ENO2, CHGA, ROBO2 | Immunofluorescence, qPCR |
