Reagent | Stock Concentration | Final Concentration | Amount |
---|---|---|---|
Fibronectin | 1 mg/mL | 5 μg/mL | 25 μL |
Laminin | 500 μg/mL | 5 μg/mL | 50 μL |
Collagen IV | 600 μg/mL | 60 μg/mL | 500 μL |
PBS, 1× | n/a | n/a | 4.5 mL |
Total | n/a | n/a | 5 mL |
n/a – not applicable
Reagent | Stock Concentration | Final Concentration | Amount |
---|---|---|---|
Fibronectin | 1 mg/mL | 5 μg/mL | 25 μL |
Laminin | 500 μg/mL | 5 μg/mL | 50 μL |
Collagen IV | 600 μg/mL | 60 μg/mL | 500 μL |
PBS, 1× | n/a | n/a | 4.5 mL |
Total | n/a | n/a | 5 mL |
n/a – not applicable