Complete Serum-free Differentiation Medium (cSFDM)

Reagent	Final Concentration	Amount
Iscove's Modified Dulbecco's Media (IMDM)	75%	375 mL
Ham’s F-12	25%	125 mL
BSA	0.75% (w/v)	3.75 mg
B-27 with RA	0.5×	5 mL
N-2	0.5×	2.5 mL
Glutamax	1×	5 mL
Ascorbic Acid	50 μg/mL	500 μL
MTG	4.5 × 10−4 M	19.55 μL
Total	n/a	500 mL

n/a – not applicable