Generation and Parallel Culture of 48 EHM in a Multi-well Format
(A) 48-well EHM plate used for the construction of EHM.
(B) EHM forming between two poles over time. The prepared hydrogel becomes opaque after 1 h (dashed boxes indicate inner and outer boundary of the hydrogel) and compacts notably by 24 h. After four weeks in culture, EHM are typically analyzed.
(C) Breaks in the hydrogel (arrow) will prevent correct EHM formation. Bar graph: 5 mm.