Table 2.
Preparation of EHM Hydrogel (Including a 10% Surplus Accounting for Pipetting Errors)
| Volume (μl) | |||
|---|---|---|---|
| EHM number: | 1 | 6 | 48 |
| Collagen (6.84 mg/mL) | 44 | 264 | 2,112 |
| 2x RPMI | 44 | 264 | 2,112 |
| 0.1 N NaOH | 6 | 36 | 288 |
| Cell mix in EHMM | 104 | 624 | 4,992 |
| Total volume | 198 | 1,188 | 9,504 |