EHM Medium (EHMM)

Reagent	Final Concentration	Volume (mL)
Iscove’s with Glutamax	n/a	500
MEM non-essential amino acid solution	n/a	5.2
B-27 supplement minus insulin	n/a	20
L-ascorbic acid 2-phosphate (300 mM)	300 μM	0.52
IGF-1 (100 μg/mL)	100 ng/mL	0.52
VEGF165 (5 μg/mL)	5 ng/mL	0.52
FGF-2 (10 μg/mL)	10 ng/mL	0.52
Total	n/a	527.8