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. 2020 Jun 3;1(1):100032. doi: 10.1016/j.xpro.2020.100032

Cardio Culture Medium (CCM)

Reagent Final Concentration Volume (mL)
RPMI 1640 with Glutamax n/a 500
Sodium pyruvate 1 mM 5.1
B-27 supplement n/a 10
L-ascorbic acid 2-phosphate (300 mM) 200 μM 0.35
Total n/a 515.45