Reagent | Final Concentration | Volume (mL) |
---|---|---|
RPMI 1640 with Glutamax | n/a | 500 |
Sodium pyruvate | 1 mM | 5.1 |
B-27 supplement | n/a | 10 |
L-ascorbic acid 2-phosphate (300 mM) | 200 μM | 0.35 |
Total | n/a | 515.45 |
Reagent | Final Concentration | Volume (mL) |
---|---|---|
RPMI 1640 with Glutamax | n/a | 500 |
Sodium pyruvate | 1 mM | 5.1 |
B-27 supplement | n/a | 10 |
L-ascorbic acid 2-phosphate (300 mM) | 200 μM | 0.35 |
Total | n/a | 515.45 |