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. 2020 Jun 3;1(1):100032. doi: 10.1016/j.xpro.2020.100032
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

Albumin, recombinant human Sigma-Aldrich Cat. #A9731
B-27 Supplement (50x) Thermo Fisher Scientific Cat. #17504044
B-27 Supplement minus insulin (50x) Thermo Fisher Scientific Cat. #A18956-01
Collagen (Acid Solubilized Telo Collagen) Collagen Solutions Cat. #FS22024
FGF-2 Peprotech Cat. #AF-100-18B
IGF-1 Peprotech Cat. #AF-100-11
L-ascorbic acid 2-phosphate Sigma-Aldrich Cat. #A8960
Matrigel (Growth Factor Reduced) BD Cat. #354230
MEM non-essential amino acid solution Thermo Fisher Scientific Cat. #11140035
RPMI powder Thermo Fisher Scientific Cat. #51800-035
Sodium pyruvate Thermo Fisher Scientific Cat. #11360070
StemPro Accutase Cell Dissociation Reagent Thermo Fisher Scientific Cat. #A1110501
TGF-β1 Peprotech Cat. #AF-100-21C
TrypLE Express Thermo Fisher Scientific Cat. #12605010
VEGF165 Peprotech Cat. #AF-100-20
Versene solution (0.48 mmol/L EDTA) Thermo Fisher Scientific Cat. #15040033
Y27632 (ROCK inhibitor) Stemgent Cat. #04-0012-10

Experimental Models: Cell Lines

HFF-1 ATCC Cat. #SCRC-1041
NHCF-V Lonza Cat. #CC-2904
HCF Promocell Cat. #C-12375

Other

48 EHM multi-well plate myriamed GmbH myrPlate