Chemicals, Peptides, and Recombinant Proteins |
|
Albumin, recombinant human |
Sigma-Aldrich |
Cat. #A9731 |
B-27 Supplement (50x) |
Thermo Fisher Scientific |
Cat. #17504044 |
B-27 Supplement minus insulin (50x) |
Thermo Fisher Scientific |
Cat. #A18956-01 |
Collagen (Acid Solubilized Telo Collagen) |
Collagen Solutions |
Cat. #FS22024 |
FGF-2 |
Peprotech |
Cat. #AF-100-18B |
IGF-1 |
Peprotech |
Cat. #AF-100-11 |
L-ascorbic acid 2-phosphate |
Sigma-Aldrich |
Cat. #A8960 |
Matrigel (Growth Factor Reduced) |
BD |
Cat. #354230 |
MEM non-essential amino acid solution |
Thermo Fisher Scientific |
Cat. #11140035 |
RPMI powder |
Thermo Fisher Scientific |
Cat. #51800-035 |
Sodium pyruvate |
Thermo Fisher Scientific |
Cat. #11360070 |
StemPro Accutase Cell Dissociation Reagent |
Thermo Fisher Scientific |
Cat. #A1110501 |
TGF-β1 |
Peprotech |
Cat. #AF-100-21C |
TrypLE Express |
Thermo Fisher Scientific |
Cat. #12605010 |
VEGF165
|
Peprotech |
Cat. #AF-100-20 |
Versene solution (0.48 mmol/L EDTA) |
Thermo Fisher Scientific |
Cat. #15040033 |
Y27632 (ROCK inhibitor) |
Stemgent |
Cat. #04-0012-10 |
|
Experimental Models: Cell Lines |
|
HFF-1 |
ATCC |
Cat. #SCRC-1041 |
NHCF-V |
Lonza |
Cat. #CC-2904 |
HCF |
Promocell |
Cat. #C-12375 |
|
Other |
|
48 EHM multi-well plate |
myriamed GmbH |
myrPlate |