Neural Induction Medium 2 (NIM2)__25 mL
| Ingredients | Final Concentration | Amount | 
|---|---|---|
| DMEM- F12 | 50% | 12 mL | 
| Neurobasal Medium | 50% | 12 mL | 
| B27 without Vitamin A (50x) | 1x | 500 μL | 
| N2-Supplement (100x) | 1x | 250 μL | 
| L-Glutamine (200 mM) | 2 mM | 250 μL | 
| CHIR 99210 (10 mM) | 3 μM | 7.5 μL | 
| Vitamin C (25 mg/mL) | 0.0625 mg/mL | 62.5 μL | 
| Purmorphamine (10 mM) | 0.5 μM | 1.25 μL |