Make 10 mL Per Sample

Reagent	Final Concentration	Volume (μL)
Sucrose (2.5 M)	440 mM	1,750
Tris-HCl (1 M, pH=7.2)	10 mM	100
KCl (1 M)	70 mM	700
MgCl2 (1 M)	5 mM	100
Spermidine (2 M)	1.5 mM	7.5
Halt Protease Inhibitors Cocktail (100×)	1×	100
RNase OUT Recombinant Ribonuclease Inhibitor (40 U/μL)	200 U/mL	50
Nuclease-free H2O	N/A	7,192.5
Total	N/A	10,000