REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-PCM1 antibody produced in rabbit	Sigma-Aldrich	Cat#HPA023374; RRID:AB_1855073
Goat anti-Rabbit IgG secondary antibody, Alexa Fluor 647	Thermo Fisher Scientific	Cat#A-21245
Chemicals, Peptides, and Recombinant Proteins
Fetal Bovine Serum	Gemini Bio Products	Cat#100-106
Halt Protease Inhibitors Cocktail (100×)	Thermo Fisher Scientific	Cat#78430
RNaseOUT Recombinant Ribonuclease Inhibitor	Invitrogen	Cat#10777019
Hoechst 33342	Invitrogen	Cat#H3570
Tris-HCl (1 M, pH=8)	Thermo Fisher Scientific	Cat#15568025
Tris-HCl (1 M, pH=7.2)	Thermo Fisher Scientific	Cat#50-843-264
Sucrose	Sigma-Aldrich	Cat#S0389
DTT	Sigma-Aldrich	Cat#3483-12-3
Red Blood Cell Lysis Solution (10×)	Promega	Cat#Z3141
Spermidine trihydrochloride	Sigma	S2501
Phosphate Buffered saline (1×)	Thermo Fisher Scientific	Cat#10010023
Experimental Models: Organisms/Strains
Mouse: ICR/CD-1	Charles River Laboratories	ICR/CD-1
Software and Algorithms
Cell Ranger v2.1	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger
Seurat v3.0	Stuart et al., 2019	https://satijalab.org/seurat/
Scrublet	Wolock et al., 2019	https://github.com/AllonKleinLab/scrublet
Other
Conical tube 15 mL	Corning	Cat#352096
40 μm Cell strainer	Corning	Cat#352340
70 μm Cell strainer	Corning	Cat#352350
Vertical rotator	N/A	N/A
Refrigerated centrifuge	N/A	N/A
Vacuum Drive Disposable Filtration System (0.22 μm)	Millipore	Cat#S2GPU05RE
Premium Microscope Slide	Fisher Scientific	Cat#22-178-277
VWR micro cover glass	VWR	Cat#48366-227